simultaneous detection of arabis mosaic virus, cherry leafroll virus and cucumber mosaic virus with coamplification of plant mrna as internal control for olive certification programs
نویسندگان
چکیده
background and aims: certification programs of plant propagating materials rely on faster, cheaper and more importantly sensitive and reliable methods for detection of systemic pathogens as indicated in national and/or international health standards of plant propagating materials. reverse transcription-polymerase chain reaction (rt-pcr) has been documented as an alternative assay for certification of plant propagating materials. rt-pcr has been shown to be necessary for olive certification due to the inefficiency of other methods in detecting viruses. the object of the present study was the optimization of a multiplex rt-pcr assay for simultaneous detection of arabis mosaic virus (armv), cherry leafroll virus (clrv) and cucumber mosaic virus (cmv) together with the plant mrna as internal control for olive certification programs in iran. materials and methods: total rna was extracted from olive tissues infected by armv, clrv and cmv as well as from healthy plants and subjected to cdna synthesis by m-mulv reverse transcriptase. simplex, duplex and multiplex rt-pcr (s-, d-, and mrt-pcr) were optimized for amplification of target genes. amplified fragments were further sequenced for evaluation of the accuracy of the assays. results: genomic fragments of armv, clrv, cmv and the plant internal control (nad 5 gene) were successfully amplified in all assays. the sequence information as well as application of the developed method on samples derived from different origins revealed the accuracy of all assays in olive certification schemes. conclusion: results from the developed s-, d-, and mrt-pcr assays revealed rt-pcr as an excellent assay for olive certification. moreover, coamplification of nad5 gene fragment suggested that was a robust marker for analyzing the accuracy of the developed rt-pcr as shown previously in other crops as well.
منابع مشابه
Simultaneous Detection of Arabis Mosaic Virus, Cherry Leafroll Virus and Cucumber Mosaic Virus with Coamplification of Plant mRNA as Internal Control for Olive Certification Programs
Background and Aims: Certification programs of plant propagating materials rely on faster, cheaper and more importantly sensitive and reliable methods for detection of systemic pathogens as indicated in national and/or international health standards of plant propagating materials. Reverse transcription-polymerase chain reaction (RT-PCR) has been documented as an alternative assay for certific...
متن کاملStudy of resistance to cucumber mosaic virus watermelon mosaic virus and zucchini yellow mosaic virus in melon cultivars
متن کامل
حذف ویروس موزاییک خیار (cucumber mosaic virus, cmv) از گلایل
ویروس موزاییک خیار از رایجترین ویروسهای گلایل در سراسر جهان است. تکثیر گلایل از طریق اندام رویشی پداژه است بنابراین بیماریهای ویروسی در آن از نسلی به نسل بعد منتقل و سبب افزایش بیماری و کاهش کیفیت آن میشود. بنابراین تولید پداژههای سالم از اهمیت زیادی برخوردار است. در این تحقیق درصد آلودگی پداژههای گلایل موجود در بازار به ویروس موزاییک خیار در دو استان تهران و البرز تعیین شد و روش گرمادرما...
An appropriate method to determine the interaction type of Cucumber mosaic virus (CMV) and Bean yellow mosaic virus (BYMV)
The occurrence of viral co-infection is a common phenomenon in cultivated and native plant species and can alter the dynamics of virus infection. In this study, disease progress was examined in single and mixed infections of Cucumber mosaic virus (CMV) and Bean yellow mosaic virus (BYMV) by measuring the rate of symptom development, disease severity and area under disease progress curve on infe...
متن کاملCharacterization of Cucumber Mosaic Virus From Mint in Tehran Province
Cucumber mosaic virus (CMV) is one of the causal agents of viral diseases that infected mint (Mentha spp.). CMV is a member of the cucumovirus genus in Bromoviridae family. The virus is naturally transmitted by aphids in a non-persistent manner.During growing season 2007-2008, leaf samples from plants of mint fields with symptoms of viral disease were collected in mint fieldas in Tehran provi...
متن کاملCharacterization of Cucumber Mosaic Virus From Mint in Tehran Province
Cucumber mosaic virus (CMV) is one of the causal agents of viral diseases that infected mint (Mentha spp.). CMV is a member of the cucumovirus genus in Bromoviridae family. The virus is naturally transmitted by aphids in a non-persistent manner.During growing season 2007-2008, leaf samples from plants of mint fields with symptoms of viral disease were collected in mint fieldas in Tehran provi...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of virologyجلد ۸، شماره ۱، صفحات ۷-۱۲
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023